Figure 1

MAFFT-based amino acid alignments of putative anticoagulant orthologues derived from the genome of Hirudo medicinalis and the respective top BLASTp hits. (A) Putative destabilase I from H. medicinalis aligned with the known sequence of the salivary bioactive protein (GenBank accession number AAA96144); (B) putative Leech Derived Tryptase Inhibitor (LDTI) from H. medicinalis aligned with the known sequence of the salivary bioactive protein (GenBank accession number AAB33769); (C) putative hirudin (HV1) from H. medicinalis aligned with the known sequence of the salivary bioactive protein (GenBank accession number APA20833); (D) putative bdellin from H. medicinalis aligned with the known sequence of the salivary bioactive protein (GenBank accession number P09865). Red boxes denote conserved cysteine residues and blue shadings represent conservation of residues between the sequences.