Figure 6

Missing-wedge correction of the cryo-EM single-molecule 3D reconstructions. (A) Two perpendicular views of a single-molecule 3D density map of an individual liposome particle reconstructed by IPET on the cryo-EM tilt series (from −57° to +60° in steps of 1.5°) after CTF correction and contrast enhancement²⁵. The 3D map was low-pass filtered at 70 Å. (B) Fourier transform of the X-Z projection of the 3D map. (C) The corresponding views of the 3D map after the 1,000 iterations of missing-wedge correction. (D) Fourier transform of the X-Z projection of the corrected 3D map. (E–H) The missing-wedge correction on a 3D map of an individual LDL particle imaged by cryo-ET and reconstructed by IPET from the tilt series (from −45° to +45° in steps of 1.5°). Two Fourier transforms of X-Z projections of the 3D maps before and after correction are shown. (I–M) The missing-wedge correction on a 3D map of an individual CETP particle (53 kDa) imaged by cryo-ET and reconstructed by IPET from the tilt series (from −49° to +65° in steps of 1.5°). Two perpendicular views and Fourier transforms of X-Z projections of the 3D maps before and after correction are shown. Scale bars: 50 nm in C and G and 10 nm in I.