Figure 1 | Scientific Reports

Figure 1

From: Highly efficient synchronization of sheep skin fibroblasts at G2/M phase and isolation of sheep Y chromosomes by flow cytometric sorting

Figure 1

Culture of sheep fibroblasts (100×). (a) Primary cells from an explant of ram ear. (b) After treating with 0.1% trypsin for 2 min, fibroblasts became round before epithelial cells. (c) Fibroblasts at 70–80% confluency. (d) Fibroblasts at 90–100% confluency. (e) Fibroblasts with a rounded shape were mitotic (black arrows) after treatment with nocodazole.

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