Figure 4 | Scientific Reports

Figure 4

From: Highly efficient synchronization of sheep skin fibroblasts at G2/M phase and isolation of sheep Y chromosomes by flow cytometric sorting

Figure 4

Use of a double thymidine block and colchicine/nocodazole treatment as a cell synchronization method. (a) Percentages (mean ± SEM) of cells arrested at the G2⁄M phase in different arresting periods, after treatment with a double thymidine (TDR) block and colchicine/nocodazole. CON: control group, which includes cells without any treatment. (b) Percentages (mean ± SEM) of cells arrested at different phases (G0/G1, S, and G2/M) of the cell cycle in different synchronization groups. TTCP: cells collected after patting dishes for double thymidine block and colchicine treatment. TTNP: cells collected after patting dishes for double thymidine block and nocodazole treatment method. CON: cells without any treatment. (c) Flow karyotype histograms of cells collected after double thymidine block and nocodazole treatment. (d) Flow karyotype histograms of cells collected after double thymidine block and colchicine treatment. (e) Flow karyotype histograms of cells without any treatment. Bars with different letters differed significantly (P < 0.05).

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