Table 1 Genes analysed and forward/reverse primer sequences used for qPCR analyses.

From: A synergic approach to enhance long-term culture and manipulation of MiaPaCa-2 pancreatic cancer spheroids

Gene symbol

Forward primer (5′->3′)

Reverse primer (5′->3′)

CD44

CACGTGGAATACACCTGCAA

GACAAGTTTTGGTGGCACG

GAPDH

CAGGAGCGAGATCCCT

GGTGCTAAGCAGTTGGT

Ki-67

GTGCAGAGAGTAACGCGG

ACACACATTGTCCTCAGCCTTC

VIMENTIN

TGCCCTTAAAGGAACCAATG

CTCAATGTCAAGGGCCATCT

TGF-ß1

AAGTGGACATCAACGGGTTC

TGCGGAAGTCAATGTACAGC

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