Figure 1
Development of a miniaturized in vitro infection model for EAV. (A) Equine dermal cells were mock infected (black dots) or infected with EAV at a MOI of 0.5 (white dots) and dispensed in 96-well culture plates. Cell viability was determined at 1 h, 24 h and 48 h post-infection. Each symbol represents an independent experiment (n = 9). The median and interquartile range (IQR) values are shown and p values were calculate using Student’s t-test. (B) EAV genome copies were determined by one-step RT-qPCR in ED cells at 1 h, 24 h, and 48 h post-infection. Each symbol represents an independent experiment (n = 6). The median and IQR values are shown. (C) Infectious particles produced by EAV-infected ED cells were quantified at 48 h post-infection on RK13 cells. Results are expressed as plaque forming units per ml of culture supernatant (PFU/ml).
