Figure 1
(A) IgM Anti-PC in cross sectional cohort, PRECISESADS. There were clear differences between different diagnoses. Low levels were observed in SLE, MCTD and SjS. The box plot for anti-PC IgM levels for all systemic rheumatic diseases and controls. Box limits indicate the 25th and 75th percentiles, centre lines indicate median values, whiskers extend 1.5 times the interquartile range from lower and upper percentiles. (B) A different profile was observed in IgM Anti-MDA. Not a clear difference between different diagnoses as for IgM anti-PC. A pairwise t-test was done for any two given diseases, orange boxes represent FDR-corrected p-values <0.005, light orange boxes represents p(FDR) < 0.05. Violin and box plots at A, B are shown for female-only data subset. The healthy control group is highlighted in green, the MCTD in red. Inset (C,D): anti-PC in MCTD – striking differences as compared to controls. For MCTD case the comparison to healthy controls is presented. Box plot shows significant decrease of anti-PC IgM in MCTD (C). No striking differences were detected for anti-MDA IgM antibodies (D). T-test p-values shown below boxplots. (E) The heatmap based on the fraction of samples for each percentile according to anti-PC and anti-MDA IgM level for MCTD and healthy controls. Numbers correspond to the following percentiles: 1 (<=10%), 2 (10–25%), 3 (25–33%), 4 (50–66%), 5 (66–75%), 6 (75–90%), 7 (>90%). Color scheme for the corresponding fraction (%) of samples is shown below.
