Table 1 Plasmids and yeast strains used in this study. Genes from Aspergillus nidulans (An), Aspergillus clavatus (Ac), Clostridium acetobutylicum (Ca), Penicillium patulum (Pp), Saccharomyces cerevisiae (Sc), Salmonella enterica serovar typhimurium (St), Treponema denticola (Td) and codon-optimized genes (opt) are indicated by prefixes in superscript.

pUG6-H

—

pBR322, hphNT1, Amp^r

²³

pRS42K

—

2 µ, kanMX, Amp^r

²⁴

pRS72N

—

2 µ, natMX, Amp^r

²⁴

pRCC-K

—

2 µ, kanMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA

²⁵

pRS42K_prpE^783Δ

pJHV1

2 µ, kanMX, Amp^r, pMET25, tCYC1, pTDH3-^StoptprpE^G783-tPGK1

This work

pJHV19

pRCC-K

2 µ, kanMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA for SFA1

This work

pJHV54

pRCC-K

2 µ, kanMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA for CIT3

This work

pJHV62

—

ColE1, Amp^R,ConL3′-pTDH3-^Tdoptter-tADH1 –ConRE‘

This work

pJHV65

—

ConLS‘-pPGK1p-^ScERG10-tVMA16 -ConR1′-ConL1′-pCCW12-^Caopthbd-tIDP –ConR2′-ConL2′-pENO2-^Caoptcrt-tPGK1 –ConR3′-ConL3′-pTDH3-^Tdoptter-tADH1 –ConRE‘-natMX-LEU2 3′Hom-KanR-ColE1-LEU 5′Hom

This work

pRS72N_ADY2

—

2 µ, natMX, Amp^r, pHXT7^-1–392-^ScADY2-tCYC1

²⁶

pRS72N_JEN1

—

2 µ, natMX, Amp^r, pHXT7^-1–392-^ScJEN1-tCYC1

²⁶

SiHV110

—

ConLS’-gfp-dropout-ConRE’-natMX-LEU2 3’Hom-KanR-ColE1-LEU 5’Hom

This work (provided by Simon Harth)

pYTK3.41

—

ColE1, Cam^R, ^Tdoptter

This work (provided by Fernando Garcés Daza)

pYTK3.43

—

ColE1, Cam^R, ^Caoptcrt

This work (provided by Fernando Garcés Daza)

pYTK3.47

—

ColE1, Cam^R, ^ScERG10

This work (provided by Fernando Garcés Daza)

pYTK3.49

—

ColE1, Cam^R, ^Caopthbd

This work (provided by Fernando Garcés Daza)

pYTK_Erg10

—

ColE1, Amp^R,ConLS‘-pPGK1-^ScERG10-tVMA16 -ConR1‘

This work (provided by Fernando Garcés Daza)

pYTK_Hbd

—

ColE1, Amp^R,ConL1′-pCCW12-^Caopthbd-tIDP –ConR2′

This work (provided by Fernando Garcés Daza)

pYTK_Crt

—

ColE1, Amp^R,ConL2′-pENO2-^Caoptcrt-tPGK1 –ConR3′

This work (provided by Fernando Garcés Daza)

pYTK01

—

ColE1, Cam^R, gfp-dropout

²⁷

pYTK05

—

ColE1, Cam^R, ConL3

²⁷

pYTK09

—

ColE1, Cam^R, TDH3p

²⁷

pYTK53

—

ColE1, Cam^R, ADH1t

²⁷

pYTK72

—

ColE1, Cam^R, ConRE

²⁷

pYTK95

—

ColE1, Cam^R, Amp^R-ColE1

²⁷

pVS06

—

CEN6ARS4, kanMX, Amp^r, pHXT7^-1–392-^ScERG10-tVMA16, pPGK1-^Caopthbd-tEFM1, pTPI1-^Caoptcrt-tYHI9, pPYK1-^Tdoptter-tIDP1, pADH1-^CaoptadhE2-tRPL3, pTDH3-^EcopteutE-tRPL41B

²²

pRS62H_ter

—

2 µ, natMX, Amp^r, pHXT7^-1–392-^Tdoptter-tFBA1

²¹

pAB02

—

2 µ, natMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA for POX1

This work (provided by Alexander Bissl)

pAB09

—

2 µ, natMX, Amp^r, pROX3-^optCas9-tCYC1, pSNR52-gRNA for CIT2

This work (provided by Alexander Bissl)

S. cerevisiae strain

Parent strain

Relevant features

Reference

CEN.PK2-1C

—

MATa leu2-3,112 ura3-52 trp1-289 his3-Δ1 MAL2–8^c SUC2

²⁸

JHY65

CEN.PK2-1C

psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1

This work

JHY162

CEN.PK2-1C

ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1

¹⁴

JHY164

CEN.PK2-1C

cit2Δ

This work

JHY174

JHY164

cit2Δ cit3Δ

This work

JHY175

CEN.PK2-1C

cit3Δ

This work

JHY179

JHY65

psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1 cit3Δ

This work

JHY180

JHY179

psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1 cit3Δ cit2Δ

This work

JHY185

JHY180

psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1 cit3Δ cit2Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1

This work

JHY194

JHY162

ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1 leu2::pPGK1-^ScERG10-tVMA16, pCCW12-^Caopthbd-tIDP, pENO2-^Caoptcrt-tPGK1, pTDH3-^Tdoptter-tADH1, pTEF-natMX-tTEF

This work

JHY196

CEN.PK2-1C

pox1Δ

This work

JHY197

JHY174

cit2Δ cit3Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1

This work

JHY211

JHY196

pox1Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1

This work

JHY212

JHY211

pox1Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1 leu2::pPGK1-^ScERG10-tVMA16, pCCW12-^Caopthbd-tIDP, pENO2-^Caoptcrt-tPGK1, pTDH3-^Tdoptter-tADH1, pTEF-natMX-tTEF

This work

JHY218

JHY65

sfa1p-sfa1Δ::TDH3p-^StoptprpE-SFA1t ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1

This work

JHY229

JHY185

psfa1-sfa1Δ::pTDH3-^StoptprpE-tSFA1 cit3Δ cit2Δ ura3::pPGK1-^PpoptMSAS-tCYC1, pHXT7^-1–392- ^AnoptnpgA-tFBA1, pFBA1-^AcoptpatG-tADH1 pacs2-acs2Δ::pTEF-hphNT1-tCYC1

This work