Figure 1

Comparison of the clearing capacity of FRUIT and hFRUIT on non-human brain samples. Comparison of murine brain samples cleared with FRUIT (a; blue) and hFRUIT (b; orange). The first four panels in a and b show 3 mm thick coronal brain sections at varying points during the clearing process. The last panels in a and b show two whole hemispheres after clearing. The heavily myelinated regions in the medial and posterior parts of the brain are generally more difficult to clear without delipidation. (grid: 1 × 1 mm). (c) typical example of autofluorescent background with 488 nm excitation in a 3 mm thick porcine brain sample cleared with hFRUIT at a depth of 315 µm at 10x magnification. (d) contrast decay of autofluorescent signal as function of depth (Costatini et al., 2015) in porcine samples cleared with FRUIT (blue) or hFRUIT (orange; n = 18 respectively; error bars indicate SEM; unpaired t-test: p = 0,0361).