Figure 3
From: hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
Effect of fixative on clearing capacity with hFRUIT. Transparency of 5 mm thick human neocortex samples fixed with formalin is limited (a). When samples are fixed with Burkhalter and Bernado’s variation of the PLP-fixation (b; Burkhalter and Bernado, 1989) the transparency is considerably improved, as shown here on 5–7 mm thick human amygdala samples. Note that both injection sites are clearly visible from the backside through the entire thickness of the sample in the left and middle panel. In this sample the thickness varies from about 7 mm (on the left side in left panel) and 5 mm on the opposite side. The second sample has a more even thickness of about 5 mm. Grid: 5 × 5 mm.
