Figure 6
From: hFRUIT: An optimized agent for optical clearing of DiI-stained adult human brain tissue
Sudan Black B staining for lipids on uncleared human brain sections (a,b) and sections of an hFRUIT cleared sample (c,d). For both conditions the abundance of lipids is evident by the dark blue-black staining. At lower magnification, the intensely stained white matter as well as radial fibre bundles are clearly distinguishable over a general light blue background (a and c). Likewise, in higher magnifications of ROIs in superficial cortical regions, individual tangential fibers can be detected in both cleared (b) and uncleared (d) sections (arrows in b and d and insets therein from indicated regions). The intensely stained small spots visible in the grey matter of both conditions are likely lipofuscin aggregates.
