Figure 3
From: Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.
Optimal number of RGs for accurate normalization based on pairwise variation (Vn/Vn + 1). The black line indicates the proposed cut-off value of 0.15, below which the inclusion of an additional RG is not required. (ASAC; all strains, all conditions. LOG/STAT; LOG/STAT cultures. LOG; only LOG cultures. HS; heat shock cultures. OS; oxidative stress with H2O2. EN; encysting cultures. Neff; strain Neff LOG/STAT/HS/OS/EN data combined).
