Figure 4
From: Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.
Relative expression of CSP21 during synchronous encystment (a) and HSP90 after oxidative stress (b) and heat shock (c) in Acanthamoeba castellanii strain Neff. First column: calculated fold-change of target gene; second column: fold change for best ranked RG (Table 4); third column: RG with most accurate calculated fold change (Supplementary Table S2); fourth column: RG with most inaccurate calculated fold change (Supplementary Table S2). Furthermore, fold changes based on normalization with the two highest ranked RGs for each algorithm are shown (CR; comprehensive ranking. NF; NormFinder. GN; geNorm. BK; BestKeeper coefficient of correlation. BKSTD; BestKeeper Standard Deviation. RF; RefFinder.) Bars represent the means and standard error of three biological replicates. Asterisks indicate a significant difference in fold change of expression compared to calculated fold changes of CSP21 and HSP90 (P < 0.05).
