Table 3 Comprehensive ranking of RGs according to employed algorithms for stability assessment (BK: BestKeeper coefficient of correlation; BKSTD: BestKeeper Standard deviation).
From: Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.
RANK | NormFinder | geNorm | BK | BKSTD | RefFinder | Compr. Ranking |
|---|---|---|---|---|---|---|
1. | HPRT | 18SQV | ACT | 18SQV | HPRT | HPRT |
2. | 18SQV | HPRT | HPRT | 18ST4 | 18SQV | 18SQV |
3. | G6PD | 18ST4 | RasC | HPRT | 18ST4 | 18ST4 |
4. | 18ST4 | RasC | PBGD | TBP | RasC | RasC |
5. | RasC | G6PD | G6PD | G6PD | G6PD/GAPDH | G6PD |
6. | GAPDH | GAPDH/TBP | 18SQV | GAPDH/RasC | GAPDH | |
7. | TBP | GAPDH | TBP | TBP | ||
8. | ACT | ACT | TBP | ACT | ACT | ACT |
9. | PBGD | PBGD | 18ST4 | PBGD | PBGD | PBGD |
