Table 4 Comprehensive ranking of RGs based on data obtained from different experimental conditions. (ASAC; all strains, all conditions. LOG/STAT; LOG/STAT cultures. LOG; only LOG cultures. HS; heat shock cultures. OS; oxidative stress with H2O2. EN; encysting cultures. Neff; strain Neff LOG/STAT/HS/OS/EN data combined).
From: Validation of reference genes for the normalization of RT-qPCR gene expression in Acanthamoeba spp.
RANK | ASAC | LOG/STAT | LOG | HS | OS | EN | Neff |
|---|---|---|---|---|---|---|---|
1. | HPRT | 18SQV | 18SQV | HPRT | HPRT | 18SQV | HPRT |
2. | 18SQV | 18ST4 | G6PD | RasC | 18ST4 | GAPDH | GAPDH |
3. | 18ST4 | HPRT | 18ST4 | 18SQV/TBP | RasC | 18ST4 | RasC |
4. | G6PD | G6PD | HPRT | GAPDH | HPRT | 18SQV | |
5. | RasC | ACT | ACT | GAPDH | TBP/18SQV | RasC | 18ST4 |
6. | TBP | PBGD | PBGD | G6PD | G6PD | TBP | |
7. | GAPDH/PBGD | TBP | RasC | 18ST4 | ACT | TBP | G6PD/PBGD |
8. | RasC | TBP | ACT | G6PD | ACT | ||
9. | ACT | GAPDH | GAPDH | PBGD | PBGD | PBGD | ACT |
