Figure 5
From: Mesenchymal Stromal Cell Bioreactor for Ex Vivo Reprogramming of Human Immune Cells
MSC bioreactor reprogramming of T cell cytokine responses. Stimulated (PHA/IL-2) PBMCs perfused in a bioreactor (+/− MSC) for 5-days were plated on a 6-well tissue culture dish for two additional days (A). Lymphocyte secreted factors (in the supernatant) were assayed using multiplex cytokine panel. Th1/Th2 specific cytokines are shown as a percentage change with MSC (9 M) treatment relative to acellular (0 M MSC) (n = 1 donor, 3 runs/donor) (B).
