Figure 7

Effect of SB77 on the expression of Collagen 1A, fibronectin and α-SMA by Immunofluorescence Analysis. Representative immunofluorescence images taken at lower and higher magnifications for (A) Collagen 1A (B) fibronectin and (C) α-SMA are shown. White box indicates the magnified region covering TM and SC for each marker. Increased Collagen 1A, fibronectin and α-SMA expression in trabecular meshwork beams and also in the inner wall of Schlemm’s canal endothelium was observed in tissues that received cyclic pulse (*). There was also a reduction in the inter trabecular spaces. The presence of SB77 significantly reduced the positivity of all markers studied with increased inter- trabecular spaces (white arrow). SC = Schlemm canal; TM = Trabecular meshwork and CB = Ciliary body. Scale bar = 50 μm.