Table 1 Summary of Results from HOCAS in response to cyclic pulsation in the presence or absence of SB77.

Experimental Setup 1 - Cyclic Pulsation and Steady state flow comparison
Donor Code	Age	Sex	Stable Baseline Before Pulse (µl/minute/mm Hg)		Pulse Duration (h)	Outflow Facility (24 h) (Normalized to Baseline)		OFR (EXP/CTL)		% Change in OF
Donor Code	Age	Sex	Control (no pulse)	Cyclic pulse	Pulse Duration (h)	Control (no pulse)	Cyclic pulse	OFR (EXP/CTL)		% Change in OF
1	75	M	0.17	0.25	8	0.94	0.88	0.95		−5.44
2	40	M	0.21	0.28	8	1.07	0.64	0.6		−39.92
3	60	M	0.08	0.19	8	0.95	0.84	0.88		−11.62
4	85	F	0.14	0.26	8	0.8	0.68	0.85		−14.92
5	72	M	0.08	0.16	8	0.77	0.58	0.76		−24.23
6	53	F	0.29	0.2	8	0.85	0.65	0.77		−22.66
						0.89 ± 0.04	0.71 ± 0.05			−19.8 ± 4.9
Experimental Setup 2 - Cyclic Pulsation in Presence or Absence of SB77
Donor Code	Age	Sex	Stable Baseline Before Pulse (µl/minute/mm Hg)		Pulse Duration (h)	Outflow Facility (24 h) (Normalized to Baseline)			% Change in OF
Donor Code	Age	Sex	Cyclic pulse	Cyclic pulse + SB77	Pulse Duration (h)	Cyclic pulse	Cyclic pulse + SB77		Cyclic pulse		Cyclic pulse + SB77
1	72	M	0.15	0.19	8	0.85	1.27		−15.35		27.01
2	85	M	0.18	0.14	8	0.89	1.17		−10.53		16.67
3	70	M	0.09	0.14	8	0.91	1.11		−8.65		11.2
4	85	F	0.27	0.12	8	0.8	1.03		−19.95		2.82
5	81	M	0.08	0.08	8	0.74	1.19		−25.9		19.44
						0.84 ± 0.06	1.15 ± 0.09		−16.1 ± 7.0		15.4 ± 9.06*

Outflow facility (OF) (μl/minute/mm Hg) was calculated as the ratio between the inflow rate (μl/minute) and the measured IOP (mm Hg). OFR was calculated using the formula: Treated/BL/Control/BL. % Change in OF was calculated using the formula: [(Treated/BL/Control/BL) -1] X100.
An average decrease in outflow facility (−19.79 ± 4.93%; n = 6) was observed in response to IOP oscillations (experimental setup1). In another set of experiments, an overall decrease in outflow facility of −16 ± 7.0% (n = 5) was observed in anterior segments those received IOP pulsations. Treatment with 50 µM SB77 caused significant enhancement in outflow facility by 15% (p < 0.05) (experimental setup 2). Data are represented as Mean ± SEM; **p < 0.005; *p < 0.05; Paired-t-test. EXP – Experiment; CTL = Control; OFR – Outflow ratio.

Search