Figure 4
Mitochondrial and lysosomal alterations in PARK2 KO neurons. (A) Longitudinally cut axon and mitochondria of control and PARK2 KO neurons at day 25 of differentiation. Lower panels show enlarged pictures of the areas marked by red boxes in the upper panels. Scale bar: 1 µm. (B) TOM20 (red) and DAPI (blue) immunofluorescence staining confirming an increase in mitochondrial abundance, especially visible in the perinuclear area of PARK2 KO neurons (marked with white arrows). Scale bar: 20 µm. (C) ROS production in control and PARK2 KO neurons. Data presented as ± SEM, n = 12, 3 independent differentiations. Student’s t-test, *p < 0.05 (D) LAMP1 (green) and DAPI (blue) immunofluorescence staining of PARK2 KO neurons and isogenic controls at day 25 after CCCP treatment (10 µM, 48 h). Scale bar: 20 µm. (E) Significant increase of the lysosomal area in both control and the PARK2 KO neurons after CCCP exposure. Data presented as mean ± SEM, n = 9, 3 independent differentiations. Two-way ANOVA, *p < 0.05, **p < 0.01, ns: not significant.
