Figure 2
From: Adipose tissue in health and disease through the lens of its building blocks
AT21 Signature Matrix: (A) Heatmap showing the correlations of signatures for the reference dataset compiled from various studies reporting isolated cell type transcriptome profiles. The dendrogram (on the left) shows the clustering within the reference dataset. The dendrogram is constructed using hierarchical clustering with 1-correlation as the distance and average linkage as the linkage criteria. The number of samples contained for each cell type (N), and the GEO accession numbers per study is shown on the right. Three groups revealed higher correlation and clustered together in the reference dataset in accordance with our expectations: Highest correlation is observed between subcutaneous and pericardial adipocytes followed by the cell types of the mesenchymal stem/stromal cell origin (osteoblasts, mesenchymal stem/stromal cells, chondrocytes and Adipose Stem/Stromal Cells - ASCs) and finally the T cell group (CD4+ T and CD8+ T cell subsets) follow. (B) Bar plots showing top ten cell type markers identified by the primary criterion and the secondary criterion of CellMaDe. Below each column conventional markers are demonstrated with their associated score. Bold font indicates the presence of the gene in the AT21 signature matrix and red colored bars indicate the value of the (primary/secondary) criterion was negative. Letters in brackets (M: membrane, E: extracellular, ME: both membrane and extracellular, or O: other) specify the gene ontology cellular location of the corresponding protein.
