Figure 1

PET-CT and PET-MR scans are perfectly registered. (A) Photograph of PET insert utilized in this study. (B) Representative images of [¹⁸F]-Florbetapir uptake (coronal, horizontal, sagittal) for WT and 5xFAD mice by PET-CT (top: CT only, middle: PET + CT, bottom: PET only). White represents highest uptake, blue represents zero uptake. Uptake was quantified within the brain as percent injected dose gram (%ID/g). 5xFAD mice had significantly greater uptake than WT mice (unpaired Student’s t-test, p = 0.0144, n = 6). (C) Representative images of [¹⁸F]-Florbetapir uptake (coronal, horizontal, sagittal) for WT and 5xFAD mice by PET-MR (top: MRI only, middle: PET + MRI, bottom: PET only). White represents highest uptake, blue represents zero uptake. Uptake was quantified within the brain as percent injected dose / gram (%ID/g). 5xFAD mice had significantly greater uptake than WT mice (unpaired Student’s t-test, p = 0.0055, n = 6). MR scans provide much higher contrast and therefore anatomical detail compared to CT scans. (D) Correlation of %ID/gm for each individual mouse calculated by PET-MR (y-axis) and PET-CT (x-axis)(R² = 0.96). All data are represented as mean ± SD. Images were analyzed using ASIpro software (Concorde Microsystems, Knoxville, TN, USA. https://www.sandersmedical.com/concordeMicro.htm) and the Inveon Research Workplace (IRW) software (Siemens Healthcare GmbH, Erlangen, Germany, https://www.siemens-healthineers.com/en-us/molecular-imaging/preclinical-imaging/preclinicalglobal-support).