Figure 3 | Scientific Reports

Figure 3

From: Hybrid PET/MRI enables high-spatial resolution, quantitative imaging of amyloid plaques in an Alzheimer’s disease mouse model

Figure 3The alternative text for this image may have been generated using AI.

Immunohistochemical analysis confirms similar regional pattern of Aβ accumulation. (A) Fluorescence microscopy of Aβ40 expression in PFA-perfused mice (WT and 5xFAD). Representative images of sagittal sections (left) and coronal sections (right) show Aβ40 expression (pink – top panel) and Aβ42 expression (green – bottom panel). Scale bars represent 2000μm (sagittal) and 1000μm (coronal). Specific regions outlined: whole brain (blue), cortex (red), hippocampus (pink), thalamus (aqua), caudate (orange), cerebellum (yellow), brainstem (green). (B) Representative images of Aβ40 plaques (top panel) and Aβ42 plaques (bottom panel) within each region of WT (left panel) and 5xFAD (right panel) mice. Scale bars represent 100μm. (C) Fluorescence area for both Aβ40 and Aβ42 quantified as a percent of total area of each region (mean ± SD) (quantification from sagittal sections: cerebellum, brainstem, thalamus) (quantification from coronal sections: cortex, hippocampus, caudate).

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