Figure 3
Overexpression and inhibition studies of miRNAs. Functional studies of miRNAs in prostate cancer cell lines. (a) Inhibitory effect on prostate cancer cell viability by single miRNA mimics and inhibitors in PC3 and DU145. Each mimic or inhibitor was compared to the corresponding control mimic or inhibitor in the same cell line. Results from alamarBlue viability assay (72 h post-transfection), plotted as mean ± SE of three independent experiments performed in triplicate. (b) Significant inhibitory effect on real-time proliferation by miR-374b-5p mimic transfections in PC3 and DU145 using the xCELLigence instrument. Results from one representative experiment performed in triplicate (three experiments in total) are plotted as mean ± SD for each time point. Student’s two-sided t-test, *p < 0.05, **p < 0.01, ***p < 0.001.
