Figure 5
Prostaglandin E2 release by neutrophils pretreated with DGAT and cPLA2 inhibitor. PGE2 quantification in the supernatant of neutrophils (2 × 105) pre-treated or not with CAY10650 (cPLA2) and A922500 (DGAT) inhibitors and followed by stimulation with Cr-LAAO (50 μg/mL), LPS (1 μg/mL; positive control) or RPMI (negative control) for 1 h at 37 °C and 5% CO2. This figure was created using images from Servier Medical Art Commons Attribution 3.0 Unported License (https://smart.servier.com) (A). Servier Medical Art by Servier is licensed under a Creative Commons Attribution 3.0 Unported License. PGE2 concentrations were quantitated by specific EIA in supernatant collected after incubation with RPMI or LPS or Cr-LAAO. The results were expressed as pg/mL of PGE2 produced and represent the mean ± S.E.M of 3 donors (B). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 compared to negative control, and #P < 0.05, ##P < 0.01, ###P < 0.001, ####P < 0.0001 compared to without pre-treatment with inhibitor (Data were presented with ANOVA followed by Tukey post-test).
