Figure 6
Validation of changes in gene expression by quantitative real-time PCR (qPCR). qPCR was performed to assess the mRNA levels of inner ear tissues. All primers were provided by QIAGEN. β-Actin served as an internal control. Data were expressed as mean ± SEM, D1 was compared with BOP1 (n = 5–6) and SC1 (n = 6), D28 was compared with BOP2 (n = 6–8) and SC2 (n = 8–10) using Student’s t test, *p < 0.05, **p < 0.01.
