Figure 1
Raman (a–d) and fluorescence (e–f) measurements of live haMSC exposed to µsPEF. The magnitude of the electric fields was 0 V/cm (black), 500 V/cm (dark blue), 750 V/cm (green), 1000 V/cm (light blue), 1250 V/cm (red) or 1500 V/cm (magenta) The other pulse parameters were fixed to 8 pulses, 100 μs and 1 Hz. (a) Mean normalized Raman signature of each group of cells in arbitrary units (a.u.). (b) Mean normalized Raman signature of each group of cells minus the mean normalized Raman signature of cells under sham exposure (0 V/cm). (c) Loading of LV1 from PLS analysis. (d) The LV1 score of each group. The percentage of variance supported by the LV1 is indicated in brackets. Three independent experiments were performed at least for each group. (e) Representative examples of Hoechst 3342 (blue) and Yo-Pro-1 (green) fluorescence images of haMSC exposed to µsPEF of 0 V/cm (I), 500 V/cm (II), 750 V/cm (III), 1000 V/cm (IV), 1250 V/cm (V) and 1500 V/cm (VI) magnitude. Scale bar = 100 μm. (f) Quantitative analysis of the Yo-Pro-1 fluorescence intensity of haMSC exposed to µsPEF. Three independent experiments were performed at least for each group. &’’_Student’s t-test: ns (non-statistically significant): p value > 5%, ****p value ≤ 0.01%.
