Figure 5

Overexpression of SNORD26 and SNORD96A. Primary HACs (n = 5 pool) transfected with SNORD26 and SNORD96A mini genes (black bars) or empty vector (white bars). (A) 24-h SNORD26, (B) 48-h SNORD26, (C) 24-h SNORD96A, (D) 48-h SNORD96A. SnoRNA, rRNAs, hypertrophic, chondrogenic and OA genes were determined by RT-qPCR. Data is presented as relative to the control condition (empty vector) and for statistical evaluation an independent samples t test was performed relative to the corresponding control condition using GraphPad Prism. The p values are indicated; *p < 0.05; **p < 0.01; ***p < 0.001. Data represents the mean value and error bars represent standard error mean.