Figure 3
From: Nitrative DNA damage in lung epithelial cells exposed to indium nanoparticles and indium ions
Time course of 8-nitroG formation in indium-treated A549 cells. (A) Fluorescent images of indium-treated A549 cells at different incubation times. A549 cells were treated with 200 ng/ml of In2O3, ITO and InCl3 at 37 °C for indicated durations. 8-NitroG was detected by immunocytochemistry as described in “Methods” section. The nucleus was stained with Hoechst 33258. Magnification, × 200. (B) Quantitative image analysis of 8-nitroG formation in indium-treated A549 cells. Staining intensities of 8-nitroG per area were analyzed with an ImageJ software. The relative intensity of the control at 2 h was set at 1. The data were expressed as means ± SD of 3–4 independent experiments. *p < 0.05, **p < 0.01 and ***p < 0.001 compared with the control by ANOVA followed by Tukey’s test.
