Figure 4
From: Nitrative DNA damage in lung epithelial cells exposed to indium nanoparticles and indium ions
Effects of iNOS and endocytosis inhibitors on indium-induced 8-nitroG formation. (A) Fluorescent images of 8-nitroG formation in indium-treated A549 cells. A549 cells were treated with 200 ng/ml of In2O3, ITO and InCl3 for 4 h at 37 °C. The cells were co-treated with 1400 W, Bay, MBCD, MDC and CytoD and 8-nitroG formation was detected by immunocytochemistry as described in “Methods” section. The nucleus was stained with Hoechst 33258. Magnification, × 200. (B) Quantitive image analysis for the effects of iNOS and endocytosis inhibitors on indium-exposed A549 cells. The staining intensity per area was quantified with an ImageJ software, and the relative intensity of the control was set at 1. The data were expressed as means ± SD of 3–4 independent experiments. **p < 0.01 versus control and #p < 0.05, ##p < 0.01 versus indium-treated cells by ANOVA followed by Tukey’s test.
