Figure 8 | Scientific Reports

Figure 8

From: A novel protective role for microRNA-3135b in Golgi apparatus fragmentation induced by chemotherapy via GOLPH3/AKT1/mTOR axis in colorectal cancer cells

Figure 8

Immunolocalization of beclin-1 and TGN38 in HCT-15 and SW-480 cancer cells. (A–B) Immunofluorescence assays using antibodies against TGN38 trans-Golgi protein (green channel) and beclin-1 autophagy protein (red channel) in control, 5-FU, and miR-3135b plus 5-FU treated cells. DAPI staining was used to visualize nuclei (blue channel). Relative quantification of the Golgi area of (C) SW-480 cells and (D) HCT-15 cells. Error bars represent standard deviation from the mean of triplicate and p values < 0.05 were considered statistically significant. NS = non-significant.

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