Figure 1 | Scientific Reports

Figure 1

From: Germ cell depletion in recipient testis has adverse effects on spermatogenesis in orthotopically transplanted testis pieces via retinoic acid insufficiency

Figure 1

Experimental procedure. Testes were collected from neonatal mice constitutively expressing EGFP and transplanted into recipient testes. Untreated recipients were designated as the control group. Some recipients (busulfan group) were administrated with busulfan at a dose of 44 mg/kg b.w. that is sufficient to deplete germ cells completely. In addition, some germ cell-depleted recipients were also administrated retinoic acid at a dose of 750 µg/body five times with an 8.5-day interval (yellow arrowheads) just prior to sacrifice (busulfan + RA group). Transplanted testis pieces were collected after around 146 days (139–154 days) and subjected for immunofluorescence staining.

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