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Figure 2

From: Ste2 receptor-mediated chemotropism of Fusarium graminearum contributes to its pathogenicity against wheat

Figure 2

Wild type F. graminearum exhibits chemotropic growth in response to wheat head-secreted peroxidases. (a) Directed hyphal growth of F. graminearum towards the ‘Roblin’ wheat head and 300 × concentrated ‘Roblin’ exudate. (b) SDS-PAGE of ‘Roblin’ exudate followed by staining with Coomassie blue. Protein bands excised and identified by mass spectrometry are labelled 1 and 2. Molecular weight markers are indicated on the left. (c) Directed hyphal growth of F. graminearum towards ‘Roblin’ exudate, either untreated (C) or inhibited with salicylhydroxamic acid (SHAM) and horseradish peroxidase (HRP) untreated (C), inhibited with SHAM, proteolyzed by proteinase K (Prot K) or boiled at 100 °C (compared to untreated control, ****P < 0.0001). Data represents the average of at least three experiments. n = 500 hyphae. Error bars represent standard deviation. Graphs were plotted using Graphpad Prism version 6.01 (https://www.graphpad.com). The figure was compiled and labelled using Adobe Illustrator CC 2015 (https://www.adobe.com).

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