Figure 3
Response of wild type F. graminearum towards HRP in chemotropism plate assay is not due to growth speed or subjective bias. (a) Representative image of length measurements and angles of counted hyphae growing towards HRP and water control. Direction of gradients of HRP and control compounds are indicated on the image. Examples of hyphal length measurements are depicted by the white lines along the hyphae. Hyphae marked NC were not counted because of large angle (> 45°) with respect to the gradient and multiple hyphae germinating from one conidia (marked with asterisk). Image contrast has been increased for clarity Using ImageJ (https://imagej.nih.gov/ij/). (b) Average length of hyphae of wild type F. graminearum towards a gradient of HRP or water control. Data is representative of two experiments. n = 300 hyphae. Error bars represent standard deviation. (c) Average cosine of hyphal angle of F. graminearum towards a gradient of HRP or water control with respect to the direction of the respective gradient. Data is representative of two experiments. n = 300 hyphae. Error bars represent standard deviation. Graphs were plotted using Graphpad Prism version 6.01 (https://www.graphpad.com). The figure was compiled and labelled using Adobe Illustrator CC 2015 (https://www.adobe.com).
