Figure 6
Cell wall integrity MAPK pathway is activated in response to HRP stimulation of F. graminearum. (a) Elements of MAPK cascade in cell wall integrity and pheromone signaling pathways in F. graminearum. MAPKs of interest to this study in each pathway are shaded in grey. Schematic of MAPK was drawn using Adobe Illustrator CC 2015 (https://www.adobe.com). (b) Representative immunoblot of MAPKs in the CWI and pheromone signaling pathways, FgMgv1 and FgGpmk1. Phospho- and total MAPK for both were probed for in an untreated control (C) and HRP-induced (HRP) condition in wild type F. graminearum. For normalization of quantification, α-tubulin was used. Molecular weights of detected proteins are indicated on the blot. Images were cropped using ImageJ (https://imagej.nih.gov/ij/). (c,d) Quantification analysis was performed using ImageJ software. The intensity of pMgv1 and pGpmk1 bands were normalized to tubulin, and the ratio of intensities of induced compared to uninduced samples were determined (compared to uninduced sample, *P < 0.05). Data represents the average of three independent experiments. Error bars represent standard deviation. Graphs were plotted using Graphpad Prism version 6.01 (https://www.graphpad.com). The figure was compiled using Adobe Illustrator CC 2015 (https://www.adobe.com).
