Figure 4

Results of the FINE algorithm of in-vivo multi-photon SHG images as a function of skin depth. (a) Location of the measurement and origin of the coordinate system. (b) Mean orientation angles \({{\bar{\theta}}}_{i}\) of the identified fiber families. (c) Amplitudes \({a}_{i}\) of each identified fiber family. (d) Dispersion parameter \({b}_{i}\) of each identified fiber family. Error bars represent the 95% confidence intervals. (e) Derived parameter AI (alignment index) and OI (orientation index) as a function of depth. (f–i) Exemplary SHG images (left) and the local fiber orientation shown in false colors (right). The color wheel shows the assignment of each color.