Figure 2

(A) Scanning electron micrographs. Native NZ9000 (left panel) and 4B0 culture (right panel): cells were grown in GM17 media without isobutanol to similar ODs and observed under equal magnification (15KX). (B) Average cell size calculated from SEM images. Graph showing a marginal increase in size of isobutanol evolved cells: average cell size (in duplicate) of native NZ9000 cells (WT rep1, WT rep2) and 40 g/l isobutanol evolved 4B0 cells (4B0 rep1, 4B0 rep2) calculated from SEM images.