Figure 1
From: Glucocorticoid receptor complexes form cooperatively with the Hsp90 co-chaperones Pp5 and FKBPs
PPH-5 enables the formation of open and closed HSP-90 complexes. (A) Sedimentation velocity AUC analysis of *GRLBDm (grey), *GRLBDm ·HSP-90 (red), *GRLBDm·HSP-90/ATPγS (navy), *GRLBDm·HSP-90·PPH-5 (yellow), *GRLBDm·HSP-90/ATPγS·PPH-5 (green) complexes. (B) Influence of AHSA-1 (green), STI-1 (petrol) and FKB-6 (navy) cofactors on the formation of *GRLBDm·HSP-90 complexes analyzed by sedimentation velocity AUC. (C) The same experimental setup with AHSA-1 (green), STI-1 (petrol) and FKB-6 (navy) was performed in the presence of ATPγS to initiate the closing reaction of HSP-90.
