Table 1 Inter-protein crosslinked peptides detected for GRLBDm·HSP-90·PPH-5 complexes.

Peptide 1	Peptide 2	Lys 1	Lys 2	MS2 scan	Hit number in scan	Hit % spectral intensity	Independent analysis
Peptide HSP-90	Peptide GR-LBD
APFDLFENKK	ELGKAIAK	326	699	24,061	30	99	✓
DSSTMGYMAAKK	QVIAAVKWAK	601	576	22,378	84	92	✓
APFDLFENKK	MTYIKELGK	326	695	27,214	84	88	✓
SKNSIK	MTYIKELGK	329	695	10,548	29	99	✓
VEKVGVSNR	WAKAIPGFR	555	579	20,877	28	96	✓
DILEKK	WAKAIPGFR	551	579	25,527	18	99	✓
Peptide HSP-90	Peptide PPH-5
DRVEVDKNDK	LHkK	624	205	5,506	26	95
MIKLGLDIGDDEIEDSAVPSSCTAEAK	QKFEAAISTDHDKK	663	147	25,861	73	99
VEVDKNDKTVK	FEAAISTDHDKkTVAETLDINAMAIEDSYDGPR	624	158	32,007	36	99
Peptide GR-LBD	Peptide PPH-5
QVIAAVKWAK	MYGFEGEVKAK	576	319	22,754	42	85	✓
WAKAIPGFR	MYGFEGEVKAK	579	319	25,375	64	99	✓

Shown are the crosslinked products detected by mass spectrometry for GRLBDm·HSP-90·PPH-5 complexes, using two different search algorithms, xMASS and pLink. Amino acid positions refer to full length, non-tagged proteins as numbered in the Uniprot database. Scan number refers to the MS2 scan with the most hits for the specified peptide. Shown are also the number of hits for this scan, the per cent spectral intensity as well as the agreement between xMASS and pLink.

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