Figure 9

The influence of dsNlPPP1Ys treated males on morphology of IRO of males (A) and their mating partners (B) and eggs (C). Fourth-instar nymphs (3-day old) N. lugens were used for dsRNA injection. IROs were dissected from the male 2 days after emergence and from the female 4 days after emergence and photographed. n > 10. (A) The thinned vas deferens observed in dsNlPPP1Ys-treated males was marked with arrow. (B) Fewer ripe banana-shaped oocytes in females mated with dsNlPPP1Ys-treated males were observed. (C) Unfertilized eggs without red eyespot in dsNlPPP1Ys-treated group. The red eyespot of fertilized eggs in dsGFP-treated control was marked with black arrow.