Table 1 Quantitative PCR primer sequences.

From: Bax and Bak jointly control survival and dampen the early unfolded protein response in pancreatic β-cells under glucolipotoxic stress

Gene

Forward (5′–3′)

Reverse (5′–3′)

Actin

GATCTGGCACCACACCTTCT

GGGGTGTTGAAGGTCTCAAA

Atf6

TGCCTTGGGAGTCAGACCTAT

GCTGAGTTGAAGAACACGAGTC

Bak

CTTTGGCTACCGTCTGGC

CAACCGCCTCTCTGTGCGA

Bax

GGAGCAGCTTGGGAGCG

AAAAGGCCCCTGTCTTCATGA

Ddit3/Chop

CTGCCTTTCACCTTGGAGAC

CGTTTCCTGGGGATGAGATA

Dnajc3/P58ipk

TCCTGGTGGACCTGCAGTACG

CTGCGAGTAATTTCTTCCCC

Edem

AAGCCCTCTGGAACTTGCG

AACCCAATGGCCTGTCTGG

Erdj4

TAAAAGCCCTGATGCTGAAGC

TCCGACTATTGGCATCCGA

Ero1

TCAGTGGACCAAGCATGATGA

TCCACATACTCAGCATCGGG

Hspa5/Bip

TCATCGGACGCACTTGGAA

CAACCACCTTGAATGGCAAGA

Ins1

GAAGTGGAGGACCCACAAGTG

ATCCACAATGCCACGCTTCT

Ins2

GAAGTGGAGGACCCACAAGTG

GATCTACAATGCCACGCTTCT

Ire1

CCGAGCCATGAGAAACAAGAA

GGGAAGCGGGAAGTGAAGTAG

Pdia6/Pdip5

AGCTCGTCAAGGATCGCCT

TATCACCTCTGCCCTGCTTTC

Wfs1

CGGGAAGAAACGGACAGAGC

CGTAGGTAGTGTTTGCCCAC

Xbp1s

GAGTCCGCAGCAGGTG

GTGTCAGAGTCCATGGGA

Back to article page