Table 2 Integrated FTIR spectra.

From: Third Harmonic Generation microscopy distinguishes malignant cell grade in human breast tissue biopsies

Wavenumber (cm−1)

Description

Grade 0

Grade I

Grade II

Grade III

1,085

Mainly from membrane phospholipids (amide III)

0.016

0.025

0.036

0.027

1,399

CH2 wagging vibration of the acyl chains (phospholipids)

0.028

0.036

0.063

0.029

1,469

CH2 bending of the acyl chains of lipids

0.322

0.529

0.540

0.550

1646

C=O stretching (lipids)

0.004

0.006

0.006

0.006

1653

C=O stretching (lipids)

0.011

0.015

0.023

0.023

1736

C=O stretching (lipids)

0.013

0.018

0.028

0.024

1,750

C=O stretching (lipids)

0.025

0.028

0.031

0.032

2,848

Stretching vibrations of CH2 and CH3 of phospholipids, cholesterol

7.903

9.952

11.270

10.250

2,873

Symmetric stretching vibration of CH3 of acyl chains (lipids)

0.008

0.032

0.0097

0.024

2,916

Stretching vibrations of CH2 and CH3 of phospholipids, cholesterol

15.441

18.979

23.237

17.992

  1. The integrated spectra peak area depicts the concentration of chemical bonds of lipids for Grade 0, Grade I, Grade II and Grade III tissue samples.
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