Figure 4
Characterization of the nature of the cuneate nucleus relay projecting relay neurons. (a) Confocal images of cuneate nucleus neurons (red) double-labeled with markers for glutaminase (purple). Quantification of the % of cuneate nucleus projecting neurons immunoreactive for glutaminase contacted by DRG fibers (left) and quantification of the number of contacts onto glutaminase positive cuneate projecting neurons (right). (b) Confocal images of cuneate nucleus projecting neurons (red) double-labeled with markers for parvalbumin (purple). Quantification of the % of cuneate nucleus projecting neurons immunoreactive for parvalbumin contacted by DRG fibers (left) and quantification of the number of contacts onto parvalbumin positive cuneate projecting neurons (right). ***: p = 0.0003 & **: p = 0.0012 (left) and *: p = 0.0209 & *: p = 0.0125 (right) (c) Confocal images of cuneate nucleus projecting neurons (red) and transgenic labeling for glycinergic neurons (GlyT2: green). Quantification of the % of cuneate nucleus projecting neurons immunoreactive for glycine contacted by DRG fibers (left) and quantification of the number of contacts onto glycine positive cuneate projecting neurons (right). ***: p = 0.0003 (left, both comparisons). Areas boxed in the low magnification images are magnified 4 times in the left inset. All DRG fibers appear yellow on the pictures. Data were tested for normality and analyzed then with a one-way ANOVA followed by Dunnett’s post-hoc test. N = 9 sections per group and n = 3 animal per group. Scale bar equal 40 µm in (a–e). For insets scale bar equals 10 µm in (a–e).
