Figure 2 | Scientific Reports

Figure 2

From: In vitro and in vivo investigation of osteogenic properties of self-contained phosphate-releasing injectable purine-crosslinked chitosan-hydroxyapatite constructs

Figure 2

(a) SEM micrographs of MC3T3-E1 cells growing on purine-crosslinked chitosan scaffolds: CS, CS25HA, CS50HA, and CS75HA at days 7 and 28, cells are pseudo-coloured in red; Mag.: 5000X and (b) magnified micrograph representing the cellular attachment of MC3T3-E1 cells. The filopodia are shown infiltrating the scaffolds, Mag.: 20 000X, scale bar = 2 μm. (c) Immunofluorescence staining of vinculin (red), actin (green) and DAPI (blue) showing the attachment of the cells onto the scaffolds’ surface. Mag.: 40X, scale bar = 30 μm.

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