Figure 6 | Scientific Reports

Figure 6

From: Effect of echinalkamide identified from Echinacea purpurea (L.) Moench on the inhibition of osteoclastogenesis and bone resorption

Figure 6The alternative text for this image may have been generated using AI.

Echinalkamide inhibits the RANKL-mediated expression of c-Fos/NFATc1. BMMs were stimulated with 100 ng/mL RANKL with 30 μM echinalkamide for the indicated periods (0, 24, 48, 72 or 96 h). (A) The effect of echinalkamide on the protein expression levels of RANKL-induced transcription factors was evaluated using western blot analysis. Actin was used as the internal control. Full blots are provided in Supplementary Fig. S01. (B, C) Total RNA was then isolated using easy blue kit, and the mRNA expression levels were evaluated using real-time PCR. GAPDH was used as the internal control. All the data were confirmed by technical replicated (n = 3). The results are presented as the mean ± SD. Values with different letters (a, b, c, d, e) are significantly different one from another (one-way ANOVA followed by Newman-Keuls multiple range test, p < 0.05).

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