Figure 5
From: Fluorescence-guided resection of tumors in mouse models of oral cancer
Hand-held confocal imaging in mice with chemically induced tumors. Mice were intravenously injected with 0.043 mg/kg of PARPi-FL and sacrificed after 90 min; excised tongues were then immediately imaged. Because this is a chemically induced mouse model, tumors grow in the tongues’ mucosal surface. FIVE2 image settings for tumor, margins and control: BP 515–575, brightness: 94%, frame rate: 1/s, 24 µm depth. (a) PARPi-FL signal indicated presence of tumor tissue, corroborated with PARP1 IHC and H&E. No nuclear signal was observed for healthy surrounding tissue, and absence of tumor was confirmed with PARP1 IHC (faint limited staining compared to tumor) and H&E. (b) No nuclear signal was observed in the control tongue, and absence of tumor was confirmed with H&E and PARP1 IHC (faint, limited staining compared to tumor). Autofluorescence from the normal keratin found in mice gustative papillae was observed. (c) The average signal intensity arising from the control tongue was not significantly different (P > 0.99) from the normal muscle around the tumor, whereas the average signal intensity generated by the tumor areas (76.0 ± 4.2 a.u.) was significantly higher when compared to the normal adjacent tongue muscle (27.0 ± 19.6 a.u., P < 0.05) and to control (average intensity: 28.4 ± 9.4 a.u., P < 0.01).
