Figure 6
From: Fluorescence-guided resection of tumors in mouse models of oral cancer
Histological analysis of PARPi-FL uptake in C57BL/6J mice with chemically induced oral lesions. (a) Confocal microscopy showed minimal specific nuclear PARPi-FL signal arising from tongues without lesions. Nuclei of the basal layer cells showed Hoechst 33,342 uptake, but not PARPi-FL. Most of the detected signal in the green channel was demonstrated to be autofluorescence (red channel). (b) Confocal microscopy also demonstrated little specific nuclear PARPi-FL signal arising from tongues with low-grade dysplasia. (c) PARPi-FL was able to identify tumor cells, showing strong overlapping signal with the Hoechst 33,342 channel, but not the red autofluorescence channel.
