Figure 3

Fxr deficiency during pregnancy causes glucose intolerance and insulin resistance. Glucose metabolism was assessed in non-pregnant (D0) and pregnant (D18) wild type (WT) and Fxr^−/− mice at gestational day 18 (or equivalent in D0 controls). a Glucose tolerance tests (GTT) were performed. D0 WT, n = 8; D18 WT, n = 6; D0 Fxr^−/−, n = 3; D18 Fxr^−/−, n = 5. b Plasma insulin concentrations after glucose challenge in D18 mice. D18 WT, n = 6; D18 Fxr^−/−, n = 5. c Insulin tolerance tests were performed on D18 mice. D18 WT, n = 8; D18 Fxr^−/−, n = 5. p < 0.05 as determined by repeated-measures two-way ANOVA followed by Bonferroni’s post-hoc test, for the following comparisons: ^a D0 WT vs D18 WT, ^b D0 Fxr^−/− vs D18 Fxr^−/−, ^c D18 WT vs D18 Fxr^−/−. d Western blot analysis of hepatic phosphorylated Akt protein at residue Ser-473 and total Akt protein. Full-length blots are presented in Supplementary Fig. S2. n = 3 per group. p < 0.01 for the following comparisons: ^avs D0 WT, ^bvs D18 WT, ^c vs D0 Fxr^−/−.