Figure 3

Effects of RF on apoptosis-related protein expression. (A) HUVECs were treated with 20 μM RF for 36 h, protein extracts were prepared, and apoptosis-associated protein-expression levels were determined. (B, C) Relative protein expression levels were estimated after quantifying the pixel densities in autoradiograms using ImageJ. Data from three independent experiments of (A) are presented as the mean ± SD; *p < 0.05, **p < 0.01, compared to the control.