Figure 7
From: The role and function of IκKα/β in monocyte impairment
Patients with infection have decreased HLA-DR expression and TNF-α production. Peripheral blood was sampled from patients during infection and after recovery, and compared to that of healthy volunteers. (A) Baseline unstimulated levels of TNF-α and IL-10 were determined by ELISA, with monocyte surface HLA-DR measured by flow cytometry. (B) Whole blood was stimulated with 100 ng/mL of LPS for 4 h and TNF-α, IL-10 and HLA-DR were measured in the same manner. (C) Experimental design for whole blood stimulation assays. (D) CD14 monocytes were isolated from whole blood and IκKα and IκKβ mRNA expression was measured with normalization to 18S. **p < 0.01, *p < 0.05, ANOVA with post-hoc Holm–Sidak test.
