Figure 2

(A) Growth curves of the P. aeruginosa lptH conditional mutant carrying the empty plasmid pME6032, pME6032 with the wild-type gene lptH or pME6032 with different mutant variants in MH at 37 °C in microtiter plates in the absence (black lines) or in the presence of 0.5% arabinose (+ ARA; red lines) or 0.5 mM IPTG (+ IPTG; green lines). Growth was measured as OD₆₀₀. Results are the mean (± SD) of three independent experiments, each one performed in triplicate. (B) Levels of LptH variants in the P. aeruginosa lptH conditional mutant carrying the different pME6032 derivatives cultured in MH supplemented with 0.5% arabinose and 0.5 mM IPTG, determined by Western blotting of whole-cell lysates (20 μg total proteins) with an anti-LptH polyclonal antibody (the empty vector used as negative control is shown in Figure S1). The housekeeping protein LptC was used as a loading control. Full-length blots are presented in Figure S5. Images are representative of three independent experiments which gave similar results.